Affinity chromatography of extracellular
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P. Fähnrich; K. Irrgang
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Article
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1984
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Springer Netherlands
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English
⚖ 385 KB
An endo-B-glucanase of C.cellulolyticum was purified by a procedure involving concanavalin A (Con A)-Sepharose chromatography and polyacrylamide gel electrophoresis (PAGE). The enzyme produced G1 and oligosaccharides from CMC. Chromatography on Procion Red HE3B-Agarose proved to be useful in the sep