✦ LIBER ✦

Induction of apoptosis in androgen-independent mouse mammary cell line by 1, 25-dihydroxyvitamin D3

✍ Scribed by Yuzo Furuya; Sho Ohta; Jun Shimazaki

Publisher: John Wiley and Sons
Year: 1996
Tongue: French
Weight: 639 KB
Volume: 68
Category: Article
ISSN: 0020-7136
DOI: 10.1002/(sici)1097-0215(19960927)68:1<143::aid-ijc25>3.0.co;2-b

No coin nor oath required. For personal study only.

✦ Synopsis

Publication of the International Union Agalnst Cancer Publication de I'lJnion Internationale Contre le Cancer Androgendependent tumors eventual to independent tumors after androgen withdrawal. ! x r e treatment for hormone-independent turnon is therefore needed. Androgemindependent CS-2 celh could grow in serum-free culture whether androgen is present in the medium or not. In the present study, the mechanism of cell death in CS-2 celh was examined after I, ZSdihydrovitunin DJ [I, 25(0HhD~] treatment. I, ZS(OmD3 has been examined as an anti-tumor agent, but its role in promoting cell death is poatly understood. Baaed upon the temporrl sequenced DNAfrrgmentation, motphologicchanges and loss of cell viability, the celk undwent apoptosh with I, 25(OHhD, treatment. No*them-blot anllr/rs was used to identify a d e s of genes whose expression per cell is enhanced during the apoptodc pathway. In the apoptotic process induced by I, ZS(OH)2DJ, mRNA expredon of testmterone-repressed pmstatic message 2, transforming growth factor p I, glucoseregulated 7&kDa protein and ulmodulin increased. Flowcytanetric anaJysis showed that I, ZS(O&DJ treatment resulted in a block in G/GI of the cell cycle. these^ results

demonstrate that androgen-independent CS-2 cells retain the ability to undergo rpoptoris by I, ZS(O&DJ. This system appears to be a good model for investigating apoptods of homone-independent cancer.

📜 SIMILAR VOLUMES

Sensitive induction of apoptosis in brea

Sensitive induction of apoptosis in breast cancer cells by a novel 1,25-dihydroxyvitamin D3 analogue shows relation to promoter selectivity

✍ Carina Danielsson; Ida S. Mathiasen; Sharon Y. James; Sepideh Nayeri; Claus Bret 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 121 KB 👁 2 views

The biologically active form of vitamin D 3 , the nuclear hormone 1a,25-dihydroxyvitamin D 3 (VD), is an important regulator of cellular growth, differentiation, and death. The hormone mediates its action through the activation of the transcription factor VDR, which is a member of the superfamily of

Phase II trial of high-dose, intermitten

Phase II trial of high-dose, intermittent calcitriol (1,25 dihydroxyvitamin D3) and dexamethasone in androgen-independent prostate cancer

✍ Donald L. Trump; Douglas M. Potter; Josephia Muindi; Adam Brufsky; Candace S. Jo 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 89 KB 👁 2 views

Positive and negative interaction of 1,2

Positive and negative interaction of 1,25-dihydroxyvitamin D3 and the retinoid CD437 in the induction of human melanoma cell apoptosis

✍ Carina Danielsson; Hans Törmä; Anders Vahlquist; Carsten Carlberg 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 French ⚖ 99 KB 👁 1 views

The natural ligands of the nuclear receptors vitamin D receptor (VDR) and retinoic acid receptor (RAR), i.e., 1␣,25dihydroxyvitamin D 3 (VD) and all-trans retinoic acid, have important effects on the proliferation, differentiation and apoptosis of a variety of malignant cells, including melanoma. Th

Biochemical significance of enhanced act

Biochemical significance of enhanced activity of fluorinated 1,25-dihydroxyvitamin D3 in human cultured cell lines

✍ Dr. John A. Eisman; Richard J. Frampton; Fiona J. McLean 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 646 KB

Hormonal responses to 1,25-dihydroxyvita

Hormonal responses to 1,25-dihydroxyvitamin D3 in cultured mouse osteoblast-like cells—modulation by changes in receptor level

✍ Theresa L. Chen; Janet M. Li; Todd Van Ye; Charlotte M. Cone; David Feldman 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 922 KB

Department of Medicine, Stanford C / / i r ~ er\fl\ S c h o o l o l Medfcfne, S t d i T / o r d Ca/itorii/,r 94 HI5 The level o i l,2.510H)IDl receptors in ( ~i l t ~i i . 4 m o u w o.;teohl,i+likt~ t O l i ) cells is modulated Iiy t h v r,itt' 01 cell ~> r o l i l c ~t ' ~l i o i i .

Effects of 1,25-dihydroxyvitamin D3 on t

Effects of 1,25-dihydroxyvitamin D3 on the distribution of androgen and vitamin D receptors in human prostate neonatal epithelial cells

✍ Eddy S. Leman; Robert H. Getzenberg 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 702 KB

## Abstract Although many studies have examined the mechanisms of 1,25‐dihydroxyvitamin D~3~ (calcitriol or 1,25 D) action in different prostate cancer cell lines, little is known regarding the influence of this steroid on the normal prostate. The presence of both VDR and AR in normal prostatic tis