## Abstract Calcium was identified by a pyroantimonateβosmium fixation technique in ram spermatozoa undergoing a spontaneous acrosome reaction induced by incubation of diluted semen at 39Β°C. Intracellular calcium was only detected in diluted spermatozoa and increased in amount and distribution over
β¦ LIBER β¦
Induction of acrosomal reaction and calcium uptake in ram spermatozoa by ionophores
β Scribed by Pazit Ben-Av; Sara Rubinstein; Haim Breitbart
- Book ID
- 115728375
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 715 KB
- Volume
- 939
- Category
- Article
- ISSN
- 0005-2736
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Ram spermatozoa treated with the ionophore A23187, to induce the acrosome reaction, were fixed in pyroantimonate-osmium in order to demonstrate calcium at an ultrastructural level. Prior to vesiculation, granules of precipitate occurred at points of contact between plasma and outer acrosomal membran
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