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Relationship between calcium binding sites and membrane fusion during the acrosome reaction induced by lonophore in ram spermatozoa

✍ Scribed by Watson, P. F. ;Plummer, J. M.

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 532 KB
Volume: 238
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402380114

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✦ Synopsis

Ram spermatozoa treated with the ionophore A23187, to induce the acrosome reaction, were fixed in pyroantimonate-osmium in order to demonstrate calcium at an ultrastructural level. Prior to vesiculation, granules of precipitate occurred at points of contact between plasma and outer acrosomal membranes, suggesting a discrete role for calcium during membrane fusion. The earliest stages of vesiculation always occurred in the region just anterior to the equatorial segment and it was this same site where a marked concentration of precipitate, indicating calcium binding sites, could be demonstrated.

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## Abstract A variety of treatment procedures was utilized to identify the origin and composition of the vesicles formed during the acrosome reaction of boar spermatozoa. Whether the acrosome reaction occurred spontaneously or was induced chemically the vesicles were hybrid vesicles composed of rou