## Abstract Cell division, neurite formation and acetylcholinesterase activity were examined in a clone (NBA~2~) of mouse neuroblastoma cells maintained for up to 120 hours in medium with pH values between 6.6 and 8.0. Growth rate decreased as pH was reduced from 7.8 to 6.6. Generation time at pH 7
Induction of Acetylcholinesterase Activity in Mouse Neuroblastoma Tissue Culture Cells
β Scribed by KATES, JOSEPH R.; WINTERTON, RADENE; SCHLESSINGER, KURT
- Book ID
- 109676739
- Publisher
- Nature Publishing Group
- Year
- 1971
- Tongue
- English
- Weight
- 501 KB
- Volume
- 229
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/229345a0
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract Previous work in our laboratory led us to postulate that N2a cells release adenosine into growth medium, where it acts at the extracellular adenosine receptors to modulate the sensitivity of the cells to the cyclic AMPβelevating effect of adenosine [Green, RD, J Pharmacol Exp Ther 201:6
Human SH-SY5Y neuroblastoma cells treated with retinoic acid, 12-0-tetradecanoyl-phorbol-13-acetate (TPA) or nerve growth factor differentiated morphologically to neuronlike cells with increased amounts of neurofilament protein and mRNA. All three effectors induced an increase in the amount of relat
A rapid method for the isolation of DNA from mouse cells grown in tissue culture is described. Cells are lysed in 0.24 M sodium phosphate, pH 6.8 buffer, containing 1% sodium dodecyl sulfate, 8 M urea, and 10m3 M ethylenediaminetetraacetic acid, and the crude lysate applied to a column of hydroxyapa