Induction of 16α-hydroxylase in cultured human lymphocytes

✍ Scribed by I. E. Muijsson; M. L. Coomes; E. T. Cantrell; D. E. Anderson; D. L. Busbee

• Book ID: 104784912
• Publisher: Springer
• Year: 1975
• Tongue: English
• Weight: 422 KB
• Volume: 13
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00485791

✦ Synopsis

Lymphocytes grown for 72 hr in the presence of mitogenic agents exhibited a nine-fold increase in 16e-hydroxylase activity over a 24-hr period following the addition of 17fi-estradiol as an inducing agent. The major metabolite of 17flestradiol produced by induced lymphocytes showed a chromatographic mobility indistinguishable from that of estriol. Enzymatic activity increased linearly with cell numbers, was inhibited by CO, and exhibited no induced increase in the presence of eycloheximide. A population survey indicated about 68% of a randomly selected Caucasian group to be essentially unindueible for 16ehydroxylase.