Induction and in vitro culture of soybean crown gall tumors
β Scribed by H. C. Pedersen; J. Christiansen; R. Wyndaele
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 754 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0721-7714
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The Ti plasmid DNA maintained in octopine-type crown gall tumor lines is variable, but always includes at least part of the Ti plasmid that maps over the region of Hind III fragment 1 of pTiB6-806. The right-hand boundary of transferred DNA (T-DNA) varies considerably among the three independent tum
We have used deletion mutagenesis to analyze a TR-DNA promoter from the octopine-type Ti plasmid pTiB6806. The promoter for the gene encoding mannopine synthase (mas) was cloned upstream of the bacterial kanamycin-resistance gene neomycin phosphotransferase II (NPT II). Bal31 deletion mutagenesis wa