The boundaries and copy numbers of Ti plasmid T-DNA vary in crown gall tumors
✍ Scribed by Merlo, D. J. ;Nutter, R. C. ;Montoya, A. L. ;Garfinkel, D. J. ;Drummond, M. H. ;Chilton, M. -D. ;Gordon, M. P. ;Nester, E. W.
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 715 KB
- Volume
- 177
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
The Ti plasmid DNA maintained in octopine-type crown gall tumor lines is variable, but always includes at least part of the Ti plasmid that maps over the region of Hind III fragment 1 of pTiB6-806. The right-hand boundary of transferred DNA (T-DNA) varies considerably among the three independent tumor lines examined; the left boundary was not located definitively. The T-DNA of two sibling clones of the same tumor line, E1 and E9, appears identical. The copy number of T-DNA in E9 tumor DNA appears higher for the right end (about 30 copies) than for the left end (approximately 1 copy).