Inducible nitric oxide synthase: Correlation with extracapsular spread and enhancement of tumor cell invasion in head and neck squamous cell carcinoma

✍ Scribed by Peter A. Brennan; Simon Dennis; David Poller; Marisol Quintero; Roberto Puxeddu; Gareth J. Thomas

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 266 KB
• Volume: 30
• Category: Article
• ISSN: 1043-3074
• DOI: 10.1002/hed.20675

✦ Synopsis

Abstract

Background

Extracapsular nodal spread is a major prognostic indicator in head and neck cancer. Nitric oxide (NO), primarily produced by the enzyme inducible NO synthase (iNOS), has a large number of actions in cancer biology, but no studies have investigated its possible role in extracapsular spread or tumor invasion.

Methods

Immunochemistry was used to study iNOS expression in 48 patients with either extracapsular or encapsulated metastasis. In vitro invasion assays were performed using H357 (an oral squamous cell carinoma cell line) using the iNOS inhibitor drug, 1400 W.

Results

iNOS expression was significantly associated with extracapsular spread, with 22/27 cases showing positive iNOS expression compared with 8/21 cases in the encapsulated group (p = .01). Invasion of H357 cells was inhibited by 1400 W at concentrations of 100 μM and 1 m__M__ (p = .002, p = .003).

Conclusion

iNOS protein seems to be associated with extracapsular spread and invasion in head and neck cancer. Further studies are required to understand this role more fully. © 2007 Wiley Periodicals, Inc. Head Neck, 2008