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Inducible Escherichia coli fermentation for increased plasmid DNA production

✍ Scribed by Aaron E. Carnes; Clague P. Hodgson; James A. Williams

Book ID
111718107
Publisher
Portland Press
Year
2006
Tongue
English
Weight
409 KB
Volume
45
Category
Article
ISSN
0885-4513

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UV irradiation of competent cells of Escherichia coli K12 produced an increase in the efficiency of transformation with plasmid DNA. This phenomenon has been called IPTE (increase in plasmid transformation efficiency) and is dependent on the activated state of the RecA protein. IPTE is independent o