Inducibility of cytochrome P-450 inAcinetobacter calcoaceticusby n-alkanes

✍ Scribed by Otmar Asperger; Annelore Naumann; Hans-Peter Kleber

• Book ID: 104779673
• Publisher: Springer
• Year: 1984
• Tongue: English
• Weight: 498 KB
• Volume: 19
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00454377

✦ Synopsis

Cytochrome P-450, detectable in n-hexadecane-grown cells ofAcinetobacter calcoaceticus, is not found during growth on complex media, sugars, or various metabolic indermediates, such as mono-or dicarboxylic acids. Cytochrome P-450 formation is observed after shifting cells from a non-hydrocarbon medium to a minimal medium with n-hexadecane as the sole source of carbon, or after addition of n-hexadecane to cultures growing on a non-inducing carbon source. The content increases with time. Induction of cytochrome P-450 is inhibited by streptomycin, chloramphenicol, and rifampicin. Besides n-hexane, other n-alkanes from n-hexane up to n-hexadecane are inducers, whereas cetyl alcohol or palmitic acid are not. The results indicate that the occurrence of cytochrome P-450 in n-alkane-induced ceils reflects a de novo protein synthesis. Regulation seems not to be governed by catabolite repression but by the presence of an inducer molecule, which is either an n-alkane or a very similar molecule.