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Isolation of the alkane inducible cytochrome P450 (P450alk) gene from the yeast Candida tropicalis

✍ Scribed by Dominique Sanglard; Chien Chen; John C. Loper

Book ID
117057695
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
753 KB
Volume
144
Category
Article
ISSN
0006-291X

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The suitability of Ca\*' ions for the precipitation of the microsomal fraction from the hydrocarbon-grown yeast Candida tropicalis was evaluated. In the final procedure the microsomes were precipitated by the addition of 16 mM CIaClr. Crude extracts obtained from cells via spheroplast lysis were cen