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Induced pluripotent stem cells generated without viral integration

โœ Scribed by Reto Eggenschwiler; Tobias Cantz

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
132 KB
Volume
49
Category
Article
ISSN
0270-9139

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โœฆ Synopsis

Pluripotent stem cells have been generated from mouse and human somatic cells by viral expression of the transcription factors Oct4, Sox2, Klf4, and c-Myc. A major limitation of this technology is the use of potentially harmful genome-integrating viruses.

Here, we generate mouse induced pluripotent stem (iPS) cells from fibroblasts and liver cells by using non-integrating adenoviruses transiently expressing Oct4, Sox2, Klf4, and c-Myc. These adenoviral iPS (adeno-iPS) cells show DNA demethylation characteristic of reprogrammed cells, express endogenous pluripotency genes, form teratomas, and contribute to multiple tissues, including the germline, in chimeric mice. Our results provide strong evidence that insertional mutagenesis is not required for in vitro reprogramming. Adenoviral reprogramming may provide an improved method for generating and studying patient-specific stem cells and for comparing embryonic stem cells and iPS cells.

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