A liquid chromatographic method using indirect conductimetric detection is proposed for the determination of low levels of organic compounds, which does not require any special functional characteristics of the analyte. The signal detected is proportional to the molar concentration of the analyte an
Indirect conductimetric detection of cyclodextrins in liquid chromatography
β Scribed by Takeuchi, Toyohide ;Miwa, Tomoo ;Hemmi, Akihide ;Maeda, Tsuneaki
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 247 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0935-6304
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β¦ Synopsis
Abstract
Indirect conductimetric detection of cyclodextrins (CDs) was investigated in liquid chromatography (LC). The mobile phase contained an electrolytic substance which maintained high background, and CDs were indirectly detected owing to a depression of the background signal due to dilution of the mobile phase by the analyte molecules. The dynamic reserve, defined as the ratio of the background to its noise level, was (1β2) Γ 10^5^, and the detection limits at S/N = 3 were 23β40 ng for CDs when a microcolumn LC system was employed.
π SIMILAR VOLUMES
## Abstract Cyclodextrins (CDs) are visualized by fluorimetric detection based on inclusion complexation with fluorescence probes in microcolumn liquid chromatography. Visualization of CDs is attributed to the fact that the fluorescence intensity of 2β__p__βtoluidinylnaphthaleneβ6βsulfonate or othe