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Increasing the synthesis/hydrolysis ratio of aminoacylase 1 by site-directed mutagenesis

✍ Scribed by Rainer Wardenga; Holger A. Lindner; Frank Hollmann; Oliver Thum; Uwe Bornscheuer

Book ID
116309140
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
745 KB
Volume
92
Category
Article
ISSN
0300-9084

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## Abstract The α‐galactosidase (AGA) from __Bifidobacterium adolescentis__ DSM 20083 has a high transglycosylation activity. The optimal conditions for this activity are pH 8, and 37Β°C. At high melibiose concentration (600 mM), approximately 64% of the enzyme–substrate encounters resulted in trans