Increased hydrophobicity of the S′1binding site in carboxypeptidase Y obtained by site-directed mutagenesis
✍ Scribed by Jakob R. Winther; Morten C. Kielland-Brandt; Klaus Breddam
- Publisher
- Springer-Verlag
- Year
- 1985
- Weight
- 976 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0105-1938
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## Abstract The S'~1~ binding pocket of carboxypeptidase Y is hydrophobic, spacious, and open to solvent, and the enzyme exhibits a preference for hydrophobic P'~1~ amino acid residues. Leu272 and Ser297, situated at the rim of the pocket, and Leu267, slightly further away, have been substituted by
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