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Increasing the stability of sacB transcript improves levansucrase production in Bacillus subtilis

✍ Scribed by J.P. Daguer; R. Chambert; M.F. Petit-Glatron

Book ID
111190592
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
198 KB
Volume
41
Category
Article
ISSN
0266-8254

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Serial increase in the thermal stability
Serial increase in the thermal stability of 3-isopropylmalate dehydrogenase from Bacillus subtilis by experimental evolution
✍ Satoshi Akanuma; Akihiko Yamagishi; Tairo Oshima; Nobuo Tanaka πŸ“‚ Article πŸ“… 1998 πŸ› Cold Spring Harbor Laboratory Press 🌐 English βš– 856 KB

## Abstract We improved the thermal stability of 3‐isopropylmalate dehydrogenase from __Bacillus subtilis__ by an in vivo evolutionary technique using an extreme thermophile, __Thermus thermophilus__, as a host cell. The __leuB__ gene encoding B. __subtilis__ 3‐isopropylmalate dehydrogenase was int