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Increasing the diffraction limit and internal order of a membrane protein crystal by dehydration

✍ Scribed by Anling Kuo; Matthew W Bowler; Jochen Zimmer; Jennifer F Antcliff; Declan A Doyle


Book ID
117704514
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
133 KB
Volume
141
Category
Article
ISSN
1047-8477

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Reproducible improvements in order and d
✍ Bowler, Matthew W. ;Montgomery, Martin G. ;Leslie, Andrew G. W. ;Walker, John E. πŸ“‚ Article πŸ“… 2006 πŸ› International Union of Crystallography 🌐 English βš– 1021 KB

Orthorhombic crystals of bovine F 1 -ATPase have been subjected to controlled dehydration. A decrease in the relative humidity surrounding the crystals to 90% reproducibly reduced their unit-cell volume by 22% (950 000 A ˚3) and improved the diffraction limit and mosaic spread of the crystals signif