✦ LIBER ✦
Reproducible improvements in order and diffraction limit of crystals of bovine mitochondrial F1-ATPase by controlled dehydration
✍ Scribed by Bowler, Matthew W. ;Montgomery, Martin G. ;Leslie, Andrew G. W. ;Walker, John E.
- Publisher
- International Union of Crystallography
- Year
- 2006
- Tongue
- English
- Weight
- 1021 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0907-4449
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✦ Synopsis
Orthorhombic crystals of bovine F 1 -ATPase have been subjected to controlled dehydration. A decrease in the relative humidity surrounding the crystals to 90% reproducibly reduced their unit-cell volume by 22% (950 000 A ˚3) and improved the diffraction limit and mosaic spread of the crystals significantly. These dehydrated crystals diffracted X-rays to 1.8 A ˚resolution at a synchrotron source, the best diffraction limit yet attained with these crystals, although radiation damage limited the resolution of a complete data set to 1.95 A ˚.