We have examined the level of expression of the SOS regulon in cells lacking DNA adenine methylase activity (darn-). Mud (Ap, lac) fusions to several SOS operons (recA, lexA, uvrA, uvrB, uvrD, sulA, dinD and dinF) were found to express higher levels of fl-galactosidase in darn-strains than in isogen
Increased UV-inducibility of SOS functions in a dam-3 mutant of Escherichia coli K12 uvrA
β Scribed by Albert Goze; Steven G. Sedgwick
- Book ID
- 113191399
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 598 KB
- Volume
- 52
- Category
- Article
- ISSN
- 0027-5107
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Involvement of UV-inducible protein(s) in repair of various E. coli K-12 cell strains has been investigated using a procedure of double UV irradiation and postincubation with chloramphenicol. From the course of dose survival curves the following conclusions concerning significance of a UV-inducible
In UV-irradiated cells of Escherichia coli K-12 a partial release of the restriction of non-modified phage 2 is observed when the cells are recA + lexA +. We show here that the induction of this restriction allevation (RA) also depends on the recBC enzyme and that the expression of RA requires prote