Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12
โ Scribed by M.G. Marinus; N.Ronald Morris
- Book ID
- 113190762
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 751 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0027-5107
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Mutation of the ruv gene of E. coli is associated with sensitivity to radiation, and filamentous growth after transient inhibition of DNA synthesis. The filamentation of ruv strains is abolished by mutations in sfiA or sfiB that prevent SOS induced inhibition of cell division, but this does not rest
We have examined the level of expression of the SOS regulon in cells lacking DNA adenine methylase activity (darn-). Mud (Ap, lac) fusions to several SOS operons (recA, lexA, uvrA, uvrB, uvrD, sulA, dinD and dinF) were found to express higher levels of fl-galactosidase in darn-strains than in isogen