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Increased sister chromatid exchange events in the human late replicating X

โœ Scribed by W. Schnedl; W. Pumberger; R. Czaker; P. Wagenbichler; H. G. Schwarzacher

Publisher
Springer
Year
1976
Tongue
English
Weight
235 KB
Volume
32
Category
Article
ISSN
0340-6717

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โœฆ Synopsis

Human female blood cultures were labeled with BrdU for detecting sister chromatid exchanges (SCEs) by the Hoechst 33258 fluorescence technique. Late labeling with 3H-thymidine and autoradiography allowed the identification of the late replicating X. The mean number of SCEs in the cells was 13. The isopycnotic X showed an exchange frequency according to its relative length in the karyotype; in the late replicating X a doubled number of SCE events was observed.

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โœ S. M. Morris; O. E. Domon; R. L. Kodell; L. J. McGarrity; D. R. Stegall ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Springer ๐ŸŒ English โš– 774 KB

To determine the relationships between the induction of specific biological responses and exposure to DNA-damaging agents, human teratocarcinoma-derived cells were exposed to either ethyl methanesulfonate or to methyl methanesulfonate, and sister chromatid exchange, cellular proliferation and relati