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Increased PHB productivity by high-cell-density fed-batch culture of Alcaligenes latus, a growth-associated PHB producer

✍ Scribed by Tsuneo Yamane; Masaaki Fukunaga; Yong Woo Lee

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 586 KB
Volume: 50
Category: Article
ISSN: 0006-3592
DOI: 10.1002/(sici)1097-0290(19960420)50:2<197::aid-bit8>3.0.co;2-h

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✦ Synopsis

Alcaligenes latus, a growth-associated PHB producer, was cultivated by a pH-stat modal fed-batch culture technique to attain high PHB productivity. Both sucrose solution and inorganic medium were fed in conjunction with the supply of ammonia solution which serves as a nitrogen source and as a means of pH control. Compositions of the inorganic medium were formulated by elemental analysis of A. latuscell mass. The effect on inoculum size was examined to reduce culture time. High concentrations of cell (142 g/L) and PHB (68.4 g/L) were obtained in a short culture time (18 h) with an inoculum size of 13.7 g/L. The PHB content and the PHB productivity at the end of the fed-batch culture were 50% of dry cell weight and 4.0 g PHB/(L. h), respectively.

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