Increased hyaluronic acid production on stimulation of DNA synthesis in chick embryo fibroblasts
β Scribed by MOSCATELLI, DAVID; RUBIN, HARRY
- Book ID
- 109694608
- Publisher
- Nature Publishing Group
- Year
- 1975
- Tongue
- English
- Weight
- 232 KB
- Volume
- 254
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/254065a0
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## Abstract Lactic acid production by chick embryo fibroblasts occurs in the absence of exogenous glucose. Fifteen to 50βfold less lactic acid is formed in the absence of glucose than in its presence. Nevertheless, serum and pH stimulation enhances this residual lactic acid production to the same r
## Abstract Stimulation of the DNA synthesis and mitoses in stationary cultures of mouse embryo fibroblastβlike cells was induced by various agents such as ribonuclease, digitonin, fresh medium and commercial preparations of hyaluronidases. Time sequence of stimulation was similar in experiments wi
## Abstract Calf serum as well as rat and mouse sera has a factor that stimulates hyaluronic acid synthesis in cultured rat fibroblasts. Such a factor was partially purified from calf serum and characterized. It has a molecular weight of approximately 150,000. The activity of the factor is lost by
## Abstract Decreasing the K^+^ concentration of the medium from 5 mM to 0.59 mM decreased the K^+^ content of chick embryo fibroblasts to 22% of control values and increased the Na^+^ content to 820% of control values. The alteration of monovalent cation content occurred within two hours but had n
## Abstract Whole serum and elevated pH previously had been found to stimulate both cell multiplication and hyaluronic acid production by chick embryo fibroblasts in culture. In a study to determine whether cell multiplication and hyaluronic acid production both respond to a single wellβdefined sub