𝔖 Bobbio Scriptorium
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Increased CD1-positive cells in peripheral blood of AIDS and ARC patients

✍ Scribed by S. Chollet-Martin; G. Pialoux; M.A. Gougerot-Pocidalo


Book ID
107906169
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
328 KB
Volume
17
Category
Article
ISSN
0165-2478

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Peripheral blood mononuclear cells from HIV-1 -infected persons were mixed with 5 M guanidine thiocyanate, and HIV-1 -specific probes were hybridized with target RNA directly in the lysate. No RNA purification was needed. Hybrids were purified by repeated capture on superparamagnetic beads coated