Increased cAMP production after short-term capacitively coupled stimulation in bovine growth plate chondrocytes

✍ Scribed by Dr. Carl T. Brighton; Peter F. Townsend

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 726 KB
• Volume: 6
• Category: Article
• ISSN: 0736-0266
• DOI: 10.1002/jor.1100060412

✦ Synopsis

Growth plate chondrocytes from newborn calf costochondral junctions grown in monolayer were subjected to a capacitive AC signal of 500 V peak to peak (P-P) at 60 kHz for 48 h and were analyzed for [3H]thymidine uptake. The stimulated chondrocytes showed a 130% greater uptake over unstimulated controls. Other newborn calf growth plate chondrocytes were stimulated at 500 V P-P at 60 kHz for 2.5, 5.0, 10.0, and 20.0 min and were analyzed for CAMP. Chondrocytes stimulated for 2.5 and 5.0 min showed a 142.8% (p < 0.05) and 394.5% (p < 0.01) increase over controls, respectively.

The chondrocytes stimulated for 10.0 and 20.0 min showed no significant difference from the controls. It is concluded that short-term exposure of growth plate chondrocytes to an appropriate capacitively coupled field stimulates cAMP production, but longer-term application of the electrical field is ineffective.