✦ LIBER ✦

Capacitively coupled electrical stimulation of bovine growth plate chondrocytes grown in pellet form

✍ Scribed by Dr. Peter F. Armstrong; Dr. Carl T. Brighton; Andrew M. Star

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 688 KB
Volume: 6
Category: Article
ISSN: 0736-0266
DOI: 10.1002/jor.1100060214

No coin nor oath required. For personal study only.

✦ Synopsis

Pellets formed from isolated bovine growth plate chondrocytes were grown in various capacitively coupled electrical fields. The signals chosen were 0, 10, 100, 250, 500, 750, 1,000, and 1,500 V peak-to-peak, 60 kHz. The effect on cell proliferation and matrix production of these different voltages was determined by [3H]thymidine and [3SS]sulfate uptake, respectively. Cyclic AMP assays were done to determine if increases in either thymidine or sulfate uptake were associated with changes in cAMP levels. Significantly increased cell proliferation occurred at 500, 750, and 1,000 V peak to peak. The calculated electric fields were 1.5 to 3.0 x V/cm. Proliferation was significantly inhibited at 1,500 V peak-to-peak with a calculated field of 4.5 x Vkm. Little if any change was seen in cAMP levels at 30 or 60 min following application of the appropriate electric signals.

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