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Increase in mitochondrial content of long-chain acyl-CoA in brown adipose tissue during cold-acclimation

โœ Scribed by Per T. Normann; Torgeir Flatmark


Book ID
115738975
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
993 KB
Volume
794
Category
Article
ISSN
0005-2760

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A method for the extraction of acyl-CoA esters from tissue, and their subsequent analysis by HPLC is described. The lipids are removed by a two-phase extraction in a chloroform/methanol/water system. The long-chain acyl-CoA esters are extracted using methanol and a high salt concentration (2 M ammon