Incorporation of Tellurocysteine into Glutathione Transferase Generates High Glutathione Peroxidase Efficiency

✍ Scribed by Xiaoman Liu; Louis A. Silks; Cuiping Liu; Morgane Ollivault-Shiflett; Xin Huang; Jing Li; Guimin Luo; Ya-Ming Hou; Junqiu Liu; Jiacong Shen

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 389 KB
• Volume: 48
• Category: Article
• ISSN: 0044-8249
• DOI: 10.1002/anie.200805365

✦ Synopsis

Abstract

A rival to native peroxidase! An existing binding site for glutathione was combined with the catalytic residue tellurocysteine by using an auxotrophic expression system to create an engineered enzyme that functions as a glutathione peroxidase from the scaffold of a glutathione transferase (see picture). The catalytic activity of the telluroenzyme in the reduction of hydroperoxides by glutathione is comparable to that of native glutathione peroxidase.magnified image