Nuclei isolated from the lower eukaryote, Euglena gracilis, incorporate labelled amino acids into hot-TCA-insoluble material under conditions of incubation similar to those used for higher eukaryotes. Optimal temperature for amino acid incorporation by Euglena nucleic is 25 ~ to 30 ~ C. Optimal pH i
Incorporation of single nucleoside triphosphates by isolated cell nuclei
β Scribed by J. Wojcierowski; Halina Antosz
- Book ID
- 102626298
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 252 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
Manganese ions can form insoluble complexes or coprecipitates with nucleoside triphosphates at high pH. We have demonstrated that nucleoside triphosphates found in precipitated complexes exhibit properties similar to those of poliribonucleotides in all steps of the Schmidt-Tannhausers procedure: insoluble in perchloric acid, trichloroacetic acid, and ethanol and soluble in 0.2 N NaOH. These observations could be applied to experimentation on RNA synthesis from nucleoside triphosphates.
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## Abstract HeLa cell mitochondria were allowed to incorporate ^3^Hβthymidine in a cell free system and the effect of ethidium bromide, cytosine arabinoside and cytosine arabinoside triphosphate on the labeling of mitochondrial DNA was studied. The labeled products, isolated by sedimentation veloci