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Incorporation of labelled amino acids by nuclei isolated fromEuglena gracilis

✍ Scribed by June R. Aprille; D. E. Buetow

Book ID: 104759134
Publisher: Springer
Year: 1974
Tongue: English
Weight: 364 KB
Volume: 97
Category: Article
ISSN: 0302-8933
DOI: 10.1007/bf00403058

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✦ Synopsis

Nuclei isolated from the lower eukaryote, Euglena gracilis, incorporate labelled amino acids into hot-TCA-insoluble material under conditions of incubation similar to those used for higher eukaryotes. Optimal temperature for amino acid incorporation by Euglena nucleic is 25 ~ to 30 ~ C. Optimal pH is 5.0. ATP stimulates incorporation only to a small extent. Evidence is presented that the product synthesized is protein.

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