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Incorporation of cholesterol into serum high and low density lipoproteins

✍ Scribed by B. Lundberg; M. Lampero; L. Suominen

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 734 KB
Volume: 31
Category: Article
ISSN: 0009-3084
DOI: 10.1016/0009-3084(82)90062-7

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✦ Synopsis

Excess cholesterol (CHL) was incorporated into serum lipoproteins by incubation with Cefite®-dispersed CHL or CHL crystals. The initial rates of uptake were 15 and 7 tool CHL/ tool lipoprotein x h -t for low (LDL) and high (HDL) density lipoprotein, respectively. Saturation values were obtained after 48 h and were 90 and 65 tool CHL/mol LDL, and 42 and 32 tool/tool tlDL using CHL on Celite and CHL crystals, respectively. Characterization of the lipoproteins showed a small change in electrophoretic mobility and an increase in molecular masses, especially after incubation with CHL on Celite. Spectroscopic methods showed only minor effects on the protein moieties. Key~v)rds. serum lipoproteins; cholesterol incorporation; lipoprotein structure.

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