Incorporation of 6-thioinosine into oligoribonucleotides
โ Scribed by Chris J. Adams; Mark A. Farrow; James B. Murray; Sharon M. Kelly; Nicholas C. Price; Peter G. Stockley
- Book ID
- 103411016
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- French
- Weight
- 340 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0040-4039
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The preparation of N(2)-phenoxylacetyl-S(6)-(2,4-dinitrophenyl)-6-thioguanosine phosphoramidite and its subsequent incorporation into oligoribonucleotides is described. The identity of the oligonucleotides was confirmed by UV spectrophotometry and nucleoside composition analysis.
A r&able prqxwation of S%yanm~y~4-th ad its incorporation into oligoribnucleoddes is reported. Deproteciion qf oligo&otuuleotidks with DBU in acetonitrile followed by methanolic amraonio allmvs the use of standard N-benwyl and N-isoburyrylp7otectedp~~~r~t~. Cleavage of hammerhead rikqvmes using GCG