INCA: Software for consort 30 analysis of flow cytometric calcium determinations

✍ Scribed by Jan F. Keij; Arjan W. Griffioen; T. H. The; Ger T. Rijkers

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 326 KB
• Volume: 10
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/cyto.990100623

✦ Synopsis

The INCA program converts Consort 30-generated fluorescence list mode data collected from Indo-1-stained cells to absolute intracellular calcium concentrations (nM Ca2+i). The calcium data are plotted vs. time, allowing the user to analyze the fractions of cells responding to a given stimulus. Converted files can be restored to disk after replacing FL1 and FL2 with time and calcium, respectively, for future analysis.