The INCA program converts Consort 30-generated fluorescence list mode data collected from Indo-1-stained cells to absolute intracellular calcium concentrations (nM Ca2+i). The calcium data are plotted vs. time, allowing the user to analyze the fractions of cells responding to a given stimulus. Conve
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ANALYSIS: Software for Graphical Analysis of Multidimensional Flow Cytometric List Mode Data
β Scribed by Tom C.Bakker Schut; Richard M.P. Doornbos; Bart G. De Grooth
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 397 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0010-4809
No coin nor oath required. For personal study only.
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Two workshops addressed the question to which degree standardization of instrument set-up and calibration, and standard list mode data analysis would reduce interlaboratory variability of flow cytometric results on prestained peripheral blood mononuclear cells (PBMC). Standard instrument set-up incl