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Inactivation of transfecting DNA by physical and chemical agents: Influence of genotypes of phage lambda and host cells

โœ Scribed by S.E. Bresler; V.L. Kalinin; V.N. Shelegedin


Book ID
116036811
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
815 KB
Volume
25
Category
Article
ISSN
0027-5107

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The transfecting activity of linear lambda DNA is 100 times higher in calcium treated E. coli K12 (lambda i434) than in non-lysogenic strains: the levels of transfection are 1-2.10(7) and 1-2.10(5) infective centers per 1 mug of lambda DNA respectively. The high efficiency of lysogenic cells transfe