## Molecular Dynamics of Subtilisin Carlsberg in Aqueous and Nonaqueous Solutions C'rystul .striictiircs huvc recently appeared ,fir the enzyme suhtilisin Carlsberg in anhydrous ut~t~[onitril~~ und in wuter. To guin u mechanistic undcmtanding ofhow the solvent environmmt Uffi'cts protein .striicti
Inactivation of subtilisin Carlsberg in surfactant and salt solutions
β Scribed by C.E. Stauffer; R.S. Treptow
- Book ID
- 115749422
- Publisher
- Elsevier Science
- Year
- 1973
- Weight
- 509 KB
- Volume
- 295
- Category
- Article
- ISSN
- 0005-2795
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π SIMILAR VOLUMES
Mixtures of commercially available tridodecylamine and its hydrochloride dissolved in hexane or toluene have been used as acid-base buffers for a suspended immobilised enzyme. The activity of subtilisin Carlsberg increases with the proportion of free amine, to a similar extent in both solvents. This
Subtilisin Carlsberg, an alkaline protease from Bacillus licheniformis, was modified with polyoxyethylene (PEG) or aerosol-OT (AOT), and the solubility, conformation, and catalytic activity of the modified subtilisins in some organic media were compared under the same conditions. The solubility of m