✦ LIBER ✦
Tridodecylamine and its hydrochloride salt: an organo-soluble buffer for subtilisin Carlsberg in non-polar organic solvents
✍ Scribed by Neil Harper; Barry D. Moore; Peter J. Halling
- Book ID
- 104210334
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- French
- Weight
- 78 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0040-4039
No coin nor oath required. For personal study only.
✦ Synopsis
Mixtures of commercially available tridodecylamine and its hydrochloride dissolved in hexane or toluene have been used as acid-base buffers for a suspended immobilised enzyme. The activity of subtilisin Carlsberg increases with the proportion of free amine, to a similar extent in both solvents. This indicates that the buffer fixes the ionisation state of the enzyme in the range appropriate for catalytic activity.