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Inactivation of Escherichia coli DnaA protein by DNA polymerase III and negative regulations for initiation of chromosomal replication

✍ Scribed by Tsutomu Katayama; Kazuhisa Sekimizu

Book ID
117457180
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
122 KB
Volume
81
Category
Article
ISSN
0300-9084

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Increased synthesis of DnaA protein, obtained with plasmids carrying the dnaA gene controlled by the heat inducible lambda pL promoter, stimulated initiation of replication from oriC about threefold. The overinitiation was determined both as an increase in copy number of a minichromosome and as an i