Inactivation by the mitotic inhibitor NY 3170 of human cells in vitro
β Scribed by Wibe, E; Oftebro, R
- Book ID
- 109834877
- Publisher
- Nature Publishing Group
- Year
- 1979
- Tongue
- English
- Weight
- 771 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0007-0920
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The effect of the two closely related drugs, the sulfone 2-(2-thenyl)sulfonyl-5-bromopyrimidine (NY 4137), and the sulfoxide 2-(2-thenyl)sulfinyl-5-bromopyrimidine (NY 4138), a sulfoxide, on the survival of cells of the human line NHIK 3025 was investigated. Cell survival was measured as the ability
The effect of 2-(2-thenyl)sulfonyl-5-bromopyrimidine (NY 4137) on cells of the human line NHIK 3025 was investigated. It was shown that NY 4137 induces cell cycle specific inhibition in metaphase. A dose-dependent prolongation of metaphase was found and the fraction of cells able to escape metaphase
## Abstract This study attempted to investigate whether different levels of mitotic activity exist within different physical regions of a human embryonic stem (hES) cell colony. Incorporation of 5βbromoβ2βdeoxyuridine (BrdU) within newlyβsynthesized DNA, followed by immunocytochemical staining was