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In vivo visualization of actin dynamics and actin interactions by BiFC

✍ Scribed by Ines Anderie; Andreas Schmid


Book ID
108084532
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
540 KB
Volume
31
Category
Article
ISSN
1065-6995

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## Abstract Previous Brownian dynamics (BD) simulations (Ouporov IG, Knull HR and Thomasson KA 1999. __Biophys. J__. 76: 17–27) of complex formation between rabbit aldolase and F‐actin have identified three lysine residues (K288, K293 and K341) on aldolase and acidic residues (DEDE) at the N‐termin

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Brownian dynamics (BD) was used to simulate the binding of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to G- and F-actin. High-resolution three-dimensional models (X-ray and homology built) of the proteins were used in the simulations. The electrostatic potential about each protein was predicte