In vivo visualization of actin dynamics and actin interactions by BiFC
β Scribed by Ines Anderie; Andreas Schmid
- Book ID
- 108084532
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 540 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1065-6995
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π SIMILAR VOLUMES
## Abstract Previous Brownian dynamics (BD) simulations (Ouporov IG, Knull HR and Thomasson KA 1999. __Biophys. J__. 76: 17β27) of complex formation between rabbit aldolase and Fβactin have identified three lysine residues (K288, K293 and K341) on aldolase and acidic residues (DEDE) at the Nβtermin
## SYNOPSIS Photon correlation spectroscopy was used to study both F-actin and F-actinlfilamin networks in solution. The measured autocorrelation functions were analyzed with the inverse Laplace transform CONTIN. The resulting frequency distributions consist of maximal five relatively narrow peaks
Brownian dynamics (BD) was used to simulate the binding of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to G- and F-actin. High-resolution three-dimensional models (X-ray and homology built) of the proteins were used in the simulations. The electrostatic potential about each protein was predicte