In vivo Tn5-based transposon mutagenesis of Streptomycetes
β Scribed by Lutz Petzke; Andriy Luzhetskyy
- Publisher
- Springer
- Year
- 2009
- Tongue
- English
- Weight
- 244 KB
- Volume
- 83
- Category
- Article
- ISSN
- 1432-0614
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Transposon mutagenesis was applied to the isolation of mutants of the facultatively methylotrophic Acetobacter methanolzcus MB58. The transposon Tn5 (pSU2011) was transferred from Escherichiu eoli SM 10 by means of conjugation to Acetobacter methanolicus MB58. Four out of 1850 stable Km-resistant tr
Drug-resistance element Tn5 coding for kanamycin resistance was used for mutagenesis of Alcaligenes eutrophus strain H16. The vehicle for introducing Tn5 into A. eutrophus was plasmid pJB4JI harboured by Escherichia coli. Kanamycin-resistant transconjugants occurred at a frequency of approximately 5