In vivo measurement of normal rat intracellular pyruvate and lactate levels after injection of hyperpolarized [1-13C]alanine
โ Scribed by Simon Hu; Minhua Zhu; Hikari A.I. Yoshihara; David M. Wilson; Kayvan R. Keshari; Peter Shin; Galen Reed; Cornelius von Morze; Robert Bok; Peder E.Z. Larson; John Kurhanewicz; Daniel B. Vigneron
- Book ID
- 113809689
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 570 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0730-725X
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Measurements of the conversion of hyperpolarized [1-13 C]pyruvate into lactate, in the reaction catalyzed by lactate dehydrogenase, have shown promise as a metabolic marker for the presence of disease and response to treatment. However, it is unclear whether this represents net flux of label from py
## Abstract [1โ^13^C]Pyruvate is a readily polarizable substrate that has been the subject of numerous magnetic resonance spectroscopy (MRS) studies of in vivo metabolism. In this work ^13^CโMRS of hyperpolarized [1โ^13^C]pyruvate was used to interrogate a metabolic pathway involved in neither aero
A single-voxel Carr-Purcell-Meibloom-Gill sequence was developed to measure localized T(2) relaxation times of (13)C-labeled metabolites in vivo for the first time. Following hyperpolarized [1-(13)C]pyruvate injections, pyruvate and its metabolic products, alanine and lactate, were observed in the l