✦ LIBER ✦

In vivo localization of a bispecific antibody which targets human t lymphocytes to lyse human colon cancer cells

✍ Scribed by Ian G. Barr; Sylvia Miescher; Vladimir Von Fliedner; Franz Buchegger; Catherine Barras; Antonio Lanzavecchia; Jean-Pierre Mach; Stefan Carrel

Publisher: John Wiley and Sons
Year: 1989
Tongue: French
Weight: 742 KB
Volume: 43
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910430327

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✦ Synopsis

A bispecific MAb was derived from the fusion of a hybridoma producing MAb CD3 with a hybridoma producing MAb L-DI (which is directed against a 41-kDa glycoprotein expressed in most gastro-intestinal and pancreatic carcinomas). Bispecific antibody molecules were isolated from parental antibody molecules by the use of hydroxylapatite-HPLC and shown to target human cytolytic T lymphocytes, irrespective of their original specificity, to specifically lyse human colon carcinoma cells. Localization studies in vivo using nude mice bearing human colon carcinoma xenografts showed significant accumulation of the HPLC-purified '251-labelled bispecific antibodies into the tumor compared to '"I-labelled control CD3 antibody.

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