Chemical synthesis and in vivo hyperexpr
β
Calogero, Raffaele A. ;Pon, Cynthia L. ;Gualerzi, Claudio O.
π
Article
π
1987
π
Springer
π
English
β 734 KB
An artificial gene encoding the Escherichia coli translational initiation factor IF1 was synthesized based on the primary structure (71 amino acid residues) of the protein. Codons for individual amino acids were selected on the basis of the preferred codon usage found in the structural genes for the