In vivo intermolecular recombination in Escherichia coli: application to plasmid constructions
โ Scribed by Eric Degryse
- Book ID
- 116133398
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 571 KB
- Volume
- 170
- Category
- Article
- ISSN
- 0378-1119
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The F' plasmids ORF-1 (purE+ tsxs proC+ lac+) and F'14 (argE+ metB+ ilv+) contain active regions of recombination, fre I and fre II correspondingly. The plasmid ORF-1 is stable in recF- cells (i.e., with the RecBC pathway of recombination) and decays in rec+ cells (RecBCF pathway) giving two types o
The heritable stability of the multicopy plasmid ColE1 and its natural relatives, requires the presence in the plasmid of a site (cer in ColE1) that acts as a substrate for site-specific recombination, thereby maintaining plasmids in the monomeric state. Multimerization, promoted by homologous recom