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Plasmid-phage recombination in T7 infected Escherichia coli

โœ Scribed by James C. Stone; R.C. Miller Jr.

Book ID
118367750
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
836 KB
Volume
137
Category
Article
ISSN
0042-6822

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## Abstract In order to release host cells from plasmidโ€mediated increases in metabolic load and high gene dosages, we developed a plasmidโ€free, T7โ€based __E. coli__ expression system in which the target gene is siteโ€specifically integrated into the genome of the host. With this system, plasmidโ€los