6000, Fluka), 100 mM CaCl 2 , 0.6 M KCl, 10 mM Tris-HCl (Sigma), pH 7.5) were added and the mixture was incubated on ice for 20 min. One milliliter of room temperature Solution E was then added and the incubation proceeded for an additional 20 min at room temperature. Aliquots of the transformation
In vivo footprinting: Studies of protein–DNA interactions in gene regulation
✍ Scribed by Peter E. Nielsen
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 536 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0265-9247
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Dysfunctional regulation and impaired expression of the FMR1 gene are responsible for the fragile X syndrome. We studied the transcriptional regulation of the normal gene and its aberrant regulation by mutation and methylation by analysis of in vivo protein/DNA contact at the promoter region [Schwem
## Abstract Exposure to environmental chemicals often induces changes in gene expression leading to a variety of developmental and physiological problems. Understanding the underlying mechanism of these changes will aid in assessing human risk to these chemicals. Traditional methods for analyzing p
## Abstract Allele‐specific transcription is a characteristic feature of imprinted genes. Many imprinted genes are also transcribed in a tissue‐ or cell type‐specific manner. Overlapping epigenetic signals must, therefore, modulate allele‐specific and tissue‐specific expression at imprinted loci. I