Twenty minutes of hypoxic hypoxia in five anesthetized rats reversibly reduced cerebral PCr and pH while ATP stayed constant. Complete metabolic and neurologic recovery occurred after oxygen was restored. Careful control of physiological parameters resulted in metabolite changes that were the same,
In vivo 31P NMR studies of orientation effects upon rat brain metabolism during mild hypoxia
β Scribed by Meng Chen; Jacob A. Stolk; Jay I. Olsen; Martin P. Schweizer
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 499 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0740-3194
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β¦ Synopsis
Abstract
Energy metabolites in rat brain under the same level of hypoxia were monitored by ^31^P NMR in both horizontal and vertical magnets. The changes in PCr, P|, and pHI in the vertical setting toward the end of hypoxia were significantly larger and the recovery in the horizontally held animals was more complete. The results demonstrated quantitatively that the stress of the alignment is superimposed on the stress of hypoxia in the vertical magnet.
π SIMILAR VOLUMES
In vivo 31P-NMR was used to investigate the basis for the inhibition of denitrification by nitrite accumulated endogenously by Pseudomonas fluorescens ATCC 17822 (biotype II) at pH 7.0. Cells were immobilized in K-carrageenan to obtain high cell concentrations in the NMR tube. Acetate and nitrate in