In vitroADP-ribosylation of chromosomal proteins of the brain of developing rats

✍ Scribed by B. R. Das; M. S. Kanungo

• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 412 KB
• Volume: 11
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00364815

✦ Synopsis

In vitro ADP-ribosylation of chromosomal proteins and its modulation by spermine, 3-aminobenzamide (3-AB) and benzamide were studied by incubating the nuclei of cerebral hemisphere of 3-, 14-and 30-day old rats with 32p-NAD § Histones get ADP-ribosylated more than the non-histone chromosomal (NHC) proteins. H1 is the major target for ADP-ribosylation. Among the nucleosomal histones, H2B is ADPribosylated most. The other core histones also get ADP-ribosylated to a lesser extent. ADP-ribosylation of both histones and NHC proteins decreases during development. Spermine stimulates, whereas 3-AB and benzamide inhibit, 32p-ADP-ribose incorporation into histories and NHC proteins. These effects decrease with development. Mild digestion of chromatin by micrococcal nuclease (MNase), EcoRI and AluI prior to ADPribosylation stimulates incorporation of 32p-ADP-ribose. The degree of stimulation decreases as development proceeds. Such alterations indicate progressive condensation of chromatin with development.